› 6b6k

X-ray diffraction
1.46Å resolution

Crystal Structure of Purine Nucleoside Phosphorylase Isoform 2 from Schistosoma mansoni in complex with 6-methyl-2,3-dihydropyridazin-3-one

Released:
DOI: 10.2210/pdb6b6k/pdb
PDB entry 6b6k coloured by chain, front view.
PDB entry 6b6k coloured by chain, side view.
PDB entry 6b6k coloured by chain, top view.
Source organism: Schistosoma mansoni
Entry authors: Faheem M, Neto JB, Collins P, Pearce NM, Valadares NF, Bird L, Pereira HM, Delft FV, Barbosa JARG

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 31.44 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0U3AGT1 (Residues: 1-287; Coverage: 100%)
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand DMS 7 x DMS
Ligand CVG 1 x CVG
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P213
Unit cell:
a: 99.7Å b: 99.7Å c: 99.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.163 0.184
Expression system: Escherichia coli BL21(DE3)

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