› 6b71

X-ray diffraction
1.52Å resolution

Crystal Structure of Purine Nucleoside Phosphorylase Isoform 2 from Schistosoma mansoni in complex with3-(4-chlorophenyl)-5H,6H-imidazo[2,1-b][1,3]thiazole

Released:
DOI: 10.2210/pdb6b71/pdb
PDB entry 6b71 coloured by chain, front view.
PDB entry 6b71 coloured by chain, side view.
PDB entry 6b71 coloured by chain, top view.
Source organism: Schistosoma mansoni
Entry authors: Faheem M, Neto JB, Collins P, Pearce NM, Valadares NF, Bird L, Pereira HM, Delft FV, Barbosa JARG

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 31.44 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0U3AGT1 (Residues: 1-287; Coverage: 100%)
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand DMS 5 x DMS
Ligand A5H 1 x A5H
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P213
Unit cell:
a: 99.97Å b: 99.97Å c: 99.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.193
Expression system: Escherichia coli BL21(DE3)

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