Structure analysis

Crystal Structure of the Mg2+/CaM:Kv7.5 (KCNQ5) AB domain complex

X-ray diffraction
2.6Å resolution
Source organism: Homo sapiens
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero dimer
Accessible surface area: 10789.69 Å2
Buried surface area: 4456.98 Å2
Dissociation area: 2,125.93 Å2
Dissociation energy (ΔGdiss): 35.68 kcal/mol
Dissociation entropy (TΔSdiss): 11.29 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-143963
Assembly 2
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Multimeric state: hetero dimer
Accessible surface area: 10536.47 Å2
Buried surface area: 4528.66 Å2
Dissociation area: 2,094.52 Å2
Dissociation energy (ΔGdiss): 34.4 kcal/mol
Dissociation entropy (TΔSdiss): 11.22 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-143963
Assembly 3
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Multimeric state: hetero dimer
Accessible surface area: 10838.34 Å2
Buried surface area: 4382.92 Å2
Dissociation area: 1,979.24 Å2
Dissociation energy (ΔGdiss): 34.71 kcal/mol
Dissociation entropy (TΔSdiss): 11.19 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-143963
Assembly 4
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Multimeric state: hetero dimer
Accessible surface area: 11237.63 Å2
Buried surface area: 4484.47 Å2
Dissociation area: 2,135.4 Å2
Dissociation energy (ΔGdiss): 36.29 kcal/mol
Dissociation entropy (TΔSdiss): 11.46 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-143963

Macromolecules

Chains: A, C, E, G
Length: 75 amino acids
Theoretical weight: 8.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR82 (Residues: 361-398, 401-545; Coverage: 7%)
  • Best match: Q9NR82-4 (Residues: 361-394)
Gene name: KCNQ5
InterPro:

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Chains: B, D, F, H
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Pfam: EF-hand domain pair
InterPro:

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