Function and Biology Details
Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-125600 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
Ig-like domain-containing protein
Molecule details ›
Chain: D
Length: 206 amino acids
Theoretical weight: 23.28 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains:
Structure domains: Immunoglobulins
Length: 206 amino acids
Theoretical weight: 23.28 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical:
Q6IRV4 (Residues: 22-123, 124-227; Coverage: 80%)
Sequence domains:
Structure domains: Immunoglobulins
Ig-like domain-containing protein
Molecule details ›
Chain: E
Length: 241 amino acids
Theoretical weight: 27.19 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains:
Structure domains: Immunoglobulins
Length: 241 amino acids
Theoretical weight: 27.19 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: K7N5M4 (Residues: 5-249; Coverage: 93%)
Sequence domains:
Structure domains: Immunoglobulins
HLA class I histocompatibility antigen, B alpha chain
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.94 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains:
Structure domains:
Length: 276 amino acids
Theoretical weight: 31.94 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: P01889 (Residues: 25-300; Coverage: 82%)
Sequence domains:
Structure domains:
Beta-2-microglobulin
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
p51 RT
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.01 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
Length: 9 amino acids
Theoretical weight: 1.01 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
- Canonical: P04585 (Residues: 880-888; Coverage: 1%)
