› 6bmi

X-ray diffraction
3.9Å resolution

Crystal Structure of GltPh R397C in complex with L-Serine

Released:
DOI: 10.2210/pdb6bmi/pdb
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PDB entry 6bmi coloured by chain, front view.
PDB entry 6bmi coloured by chain, side view.
PDB entry 6bmi coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Structural characterisation reveals insights into substrate recognition by the glutamine transporter ASCT2/SLC1A5.
Scopelliti AJ, Font J, Vandenberg RJ, Boudker O, Ryan RM
OpenAccess logo Nat Commun 9 38 (2018)
PMID: 29295993

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate transporter homolog Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 420 amino acids
Theoretical weight: 44.26 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59010 (Residues: 1-417; Coverage: 98%)
Gene name: PH1295
Sequence domains: Sodium:dicarboxylate symporter family
Structure domains: Sodium:dicarboxylate symporter

Ligands and Environments

2 bound ligands:
Ligand NA 4 x NA
Ligand SER 3 x SER
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P61
Unit cell:
a: 114.75Å b: 114.75Å c: 322.336Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.227 0.248
Expression system: Escherichia coli

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Citations

9 review citations

The Human SLC1A5 (ASCT2) Amino Acid Transporter: From Function to Structure and Role in Cell Biology.
Scalise et al. (2018)
8 more

3 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
2 more

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