› 6bnz

X-ray diffraction
1.45Å resolution

Crystal structure of E144Q-glyoxalase I mutant from Zea mays in space group P4(1)2(1)2

Released:
DOI: 10.2210/pdb6bnz/pdb
PDB entry 6bnz coloured by chain, front view.
PDB entry 6bnz coloured by chain, side view.
PDB entry 6bnz coloured by chain, top view.
Source organism: Zea mays
Primary publication:
Deciphering the number and location of active sites in the monomeric glyoxalase I of Zea mays.
González JM, Agostini RB, Alvarez CE, Klinke S, Andreo CS, Campos-Bermudez VA
FEBS J 286 3255-3271 (2019)
PMID: 30993890

Function and Biology Details

Reaction catalysed: 
(R)-S-lactoylglutathione = glutathione + methylglyoxal
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110385 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactoylglutathione lyase Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 32.96 KDa
Source organism: Zea mays
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B6TPH0 (Residues: 26-315; Coverage: 92%)
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments


Cofactor: Ligand GSH 1 x GSH
2 bound ligands:
Ligand CO 1 x CO
Ligand FMT 5 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P41212
Unit cell:
a: 69.89Å b: 69.89Å c: 119.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.231
Expression system: Escherichia coli BL21

Quick links

Citations

1 mention without citation

Deciphering the number and location of active sites in the monomeric glyoxalase I of Zea mays.
González et al. (2019)