› 6bqo

X-ray diffraction
2.8Å resolution

Structure of a dual topology fluoride channel with monobody S8

Released:
DOI: 10.2210/pdb6bqo/pdb
PDB entry 6bqo coloured by chain, front view.
PDB entry 6bqo coloured by chain, side view.
PDB entry 6bqo coloured by chain, top view.
Source organisms:
Primary publication:
Cork-in-Bottle Occlusion of Fluoride Ion Channels by Crystallization Chaperones.
McIlwain BC, Newstead S, Stockbridge RB
OpenAccess logo Structure 26 635-639.e1 (2018)
PMID: 29526432

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-166476 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fluoride-specific ion channel FluC Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 13.29 KDa
Source organism: Bordetella pertussis Tohama I
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7VYU0 (Residues: 1-128; Coverage: 100%)
Gene names: BP1217, crcB, fluC
Sequence domains: CrcB-like protein, Camphor Resistance (CrcB)
Monobody S8 Chain: C
Molecule details ›
Chain: C
Length: 95 amino acids
Theoretical weight: 10.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand F 2 x F
Ligand NA 1 x NA
Ligand OLC 3 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 114.92Å b: 39.85Å c: 109.87Å
α: 90° β: 107.39° γ: 90°
R-values:
R R work R free
0.223 0.22 0.275
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Development and Differentiation in Monobodies Based on the Fibronectin Type 3 Domain.
Chandler et al. (2020)
3 more

1 mention without citation

Cork-in-Bottle Occlusion of Fluoride Ion Channels by Crystallization Chaperones.
McIlwain et al. (2018)