› 6bqu

X-ray diffraction
2.5Å resolution

Human GR (418-507) in complex with monomeric DNA binding site

Released:
DOI: 10.2210/pdb6bqu/pdb
PDB entry 6bqu coloured by chain, front view.
PDB entry 6bqu coloured by chain, side view.
PDB entry 6bqu coloured by chain, top view.
Source organisms:
Entry author: Pufall MA

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-510199 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Glucocorticoid receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 72 amino acids
Theoretical weight: 8.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P04150 (Residues: 421-490; Coverage: 9%)
Gene names: GRL, NR3C1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(P*TP*GP*CP*AP*AP*AP*TP*GP*TP*AP*CP*TP*AP*GP*CP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*AP*AP*GP*CP*TP*AP*GP*TP*AP*CP*AP*TP*TP*TP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 38.231Å b: 88.235Å c: 103.905Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.249
Expression systems:
  • Escherichia coli BL21
  • Not provided

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