› 6bqw

Electron Microscopy
4.2Å resolution

AlfA Filament bound to AMPPNP

Released:
DOI: 10.2210/pdb6bqw/pdb
PDB entry 6bqw coloured by chain, front view.
PDB entry 6bqw coloured by chain, side view.
PDB entry 6bqw coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Cryo-EM structure of the bacterial actin AlfA reveals unique assembly and ATP-binding interactions and the absence of a conserved subdomain.
Usluer GD, DiMaio F, Yang SK, Hansen JM, Polka JK, Mullins RD, Kollman JM
Proc Natl Acad Sci U S A 115 3356-3361 (2018)
PMID: 29440491
Related structures: EMD-7134

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin-like protein N-terminal domain-containing protein Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 275 amino acids
Theoretical weight: 31.15 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O52947 (Residues: 1-275; Coverage: 100%)
Sequence domains: Actin like proteins N terminal domain

Ligands and Environments

1 bound ligand:
Ligand ANP 9 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-7134
Expression system: Escherichia coli

Quick links

Citations

1 review citation

StarMap: a user-friendly workflow for Rosetta-driven molecular structure refinement.
Lugmayr et al. (2023)
4 other articles

1 mention without citation

Cryo-EM structure of the bacterial actin AlfA reveals unique assembly and ATP-binding interactions and the absence of a conserved subdomain.
Usluer et al. (2018)