› 6c59

X-ray diffraction
2.03Å resolution

Chimeric Pol kappa RIR Rev1 C-terminal domain

Released:
DOI: 10.2210/pdb6c59/pdb
PDB entry 6c59 coloured by chain, front view.
PDB entry 6c59 coloured by chain, side view.
PDB entry 6c59 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A Small Molecule Targeting Mutagenic Translesion Synthesis Improves Chemotherapy.
Wojtaszek JL, Chatterjee N, Najeeb J, Ramos A, Lee M, Bian K, Xue JY, Fenton BA, Park H, Li D, Hemann MT, Hong J, Walker GC, Zhou P
Cell 178 152-159.e11 (2019)
PMID: 31178121

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187633 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase kappa; DNA repair protein REV1 Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.77 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9QUG2 (Residues: 564-577; Coverage: 2%)
  • Canonical: Q920Q2 (Residues: 1150-1249; Coverage: 8%)
Gene names: Dinb1, Polk, Rev1, Rev1l
Sequence domains: DNA repair protein REV1 C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 47.22Å b: 47.22Å c: 89.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.219
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Mechanisms of Multidrug Resistance in Cancer Chemotherapy.
Bukowski et al. (2020)
22 more

1 mention without citation

A Small Molecule Targeting Mutagenic Translesion Synthesis Improves Chemotherapy.
Wojtaszek et al. (2019)