6c66

Electron Microscopy
3.66Å resolution

CRISPR RNA-guided surveillance complex, pre-nicking

Released:
Source organism: Thermobifida fusca YX
Related structures: EMD-7347

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-119652 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (9 distinct):
HD Cas3-type domain-containing protein Chain: G
Molecule details ›
Chain: G
Length: 944 amino acids
Theoretical weight: 104.04 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47PJ0 (Residues: 1-944; Coverage: 100%)
Gene name: Tfu_1593
Sequence domains:
CRISPR-associated protein, Cse1 family Chain: A
Molecule details ›
Chain: A
Length: 549 amino acids
Theoretical weight: 61.43 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47PJ1 (Residues: 1-549; Coverage: 100%)
Gene name: Tfu_1592
Sequence domains: CRISPR-associated protein Cse1 (CRISPR_cse1)
CRISPR-associated protein, Cse4 family Chains: B, C, D, E, F, H
CRISPR-associated protein, Cse2 family Chains: I, K
CRISPR-associated protein, Cas5e family Chain: M
CRISPR-associated protein, Cse3 family Chain: O
Target strand Chain: L
Nontarget strand Chain: N
crRNA Chain: J

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.66Å
Relevant EMDB volumes: EMD-7347
Expression system: Escherichia coli