› 6c6b

X-ray diffraction
2Å resolution

Co-crystal structure of adenylyl-sulfate kinase from Cryptococcus neoformans bound to ADP

Released:
DOI: 10.2210/pdb6c6b/pdb
PDB entry 6c6b coloured by chain, front view.
PDB entry 6c6b coloured by chain, side view.
PDB entry 6c6b coloured by chain, top view.
Source organism: Cryptococcus neoformans var. grubii H99
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylyl-sulfate kinase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 211 amino acids
Theoretical weight: 23.3 KDa
Source organism: Cryptococcus neoformans var. grubii H99
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: J9VMZ3 (Residues: 1-203; Coverage: 100%)
Gene name: CNAG_02202
Sequence domains: Adenylylsulphate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ADP 6 x ADP
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P31
Unit cell:
a: 86.33Å b: 86.33Å c: 160.81Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.224 0.267
Expression system: Escherichia coli BL21(DE3)

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