› 6c74

X-ray diffraction
1.36Å resolution

Crystal Structure of Murine CD300lf in complex with phosphocholine

Released:
DOI: 10.2210/pdb6c74/pdb
PDB entry 6c74 coloured by chain, front view.
PDB entry 6c74 coloured by chain, side view.
PDB entry 6c74 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for murine norovirus engagement of bile acids and the CD300lf receptor.
Nelson CA, Wilen CB, Dai YN, Orchard RC, Kim AS, Stegeman RA, Hsieh LL, Smith TJ, Virgin HW, Fremont DH
OpenAccess logo Proc Natl Acad Sci U S A 115 E9201-E9210 (2018)
PMID: 30194229

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CMRF35-like molecule 1 Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 12.55 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6SJQ7 (Residues: 20-128; Coverage: 34%)
  • Best match: Q6SJQ7-2 (Residues: 20-130)
Gene names: Cd300lf, Clm1, Lmir3
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand PC 1 x PC
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 32.64Å b: 40.058Å c: 68.291Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.165 0.189
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Norovirus Attachment and Entry.
Graziano et al. (2019)
20 more

2 mentions without citation

Structural basis for murine norovirus engagement of bile acids and the CD300lf receptor.
Nelson et al. (2018)
1 more