› 6cdw

X-ray diffraction
1.84Å resolution

Structure of the HAD domain of effector protein Lem4 (lpg1101) from Legionella pneumophila (inactive mutant)

Released:
DOI: 10.2210/pdb6cdw/pdb
PDB entry 6cdw coloured by chain, front view.
PDB entry 6cdw coloured by chain, side view.
PDB entry 6cdw coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Primary publication:
Legionella pneumophila effector Lem4 is a membrane-associated protein tyrosine phosphatase.
Beyrakhova K, Li L, Xu C, Gagarinova A, Cygler M
J Biol Chem 293 13044-13058 (2018)
PMID: 29976756

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DrrA phosphatidylinositol 4-phosphate binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 24.7 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZWI4 (Residues: 6-218; Coverage: 66%)
Gene name: lpg1101

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P4212
Unit cell:
a: 105.73Å b: 105.73Å c: 37.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.214
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Molecular Mimicry: a Paradigm of Host-Microbe Coevolution Illustrated by Legionella.
Mondino et al. (2020)
2 more

1 mention without citation

Legionella pneumophila effector Lem4 is a membrane-associated protein tyrosine phosphatase.
Beyrakhova et al. (2018)