› 6cf3

X-ray diffraction
1.12Å resolution

Ethylene forming enzyme Y306A variant in complex with manganese and 2-oxoglutarate

Released:
DOI: 10.2210/pdb6cf3/pdb
PDB entry 6cf3 coloured by chain, front view.
PDB entry 6cf3 coloured by chain, side view.
PDB entry 6cf3 coloured by chain, top view.
Source organism: Pseudomonas savastanoi pv. phaseolicola
Primary publication:
Structural, Spectroscopic, and Computational Insights from Canavanine-Bound and Two Catalytically Compromised Variants of the Ethylene-Forming Enzyme.
Chatterjee S, Fellner M, Rankin J, Thomas MG, J S Rifayee SB, Christov CZ, Hu J, Hausinger RP
OpenAccess logo Biochemistry (2024)
PMID: 38577885

Function and Biology Details

Reactions catalysed: 
2-oxoglutarate + O(2) = ethylene + 3 CO(2) + H(2)O
(1a) 2-oxoglutarate + L-arginine + O(2) = succinate + CO(2) + 5-hydroxy-L-arginine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152192 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-oxoglutarate-dependent ethylene/succinate-forming enzyme Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 39.62 KDa
Source organism: Pseudomonas savastanoi pv. phaseolicola
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32021 (Residues: 1-350; Coverage: 100%)
Gene name: efe
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MN 1 x MN
Ligand AKG 1 x AKG
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Unit cell:
a: 44.37Å b: 85.41Å c: 87.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.148 0.167
Expression system: Escherichia coli BL21(DE3)

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