› 6chj

X-ray diffraction
2.43Å resolution

Wax ester synthase/diacylglycerol acyltransferase from Marinobacter aquaeolei VT8

Released:
DOI: 10.2210/pdb6chj/pdb
PDB entry 6chj coloured by chain, front view.
PDB entry 6chj coloured by chain, side view.
PDB entry 6chj coloured by chain, top view.
Source organism: Marinobacter nauticus VT8
Primary publication:
Structural and Biochemical Studies of a Biocatalyst for the Enzymatic Production of Wax Esters.
Petronikolou N, Nair SK
ACS Catal 8 6334-6344 (2018)
PMID: 31559109

Function and Biology Details

Reaction catalysed: 
Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106709 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diacylglycerol O-acyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 455 amino acids
Theoretical weight: 51.54 KDa
Source organism: Marinobacter nauticus VT8
Expression system: Escherichia coli
UniProt:
  • Canonical: A1TX06 (Residues: 1-455; Coverage: 100%)
Gene name: Maqu_0168
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 69.268Å b: 103.905Å c: 69.529Å
α: 90° β: 103.51° γ: 90°
R-values:
R R work R free
0.215 0.211 0.279
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The production of wax esters in transgenic plants: 
towards a sustainable source of bio-lubricants.
Domergue et al. (2022)
2 more

2 mentions without citation

Structural and Biochemical Studies of a Biocatalyst for the Enzymatic Production of Wax Esters.
Petronikolou et al. (2018)
1 more