› 6ci0

X-ray diffraction
2.4Å resolution

Catalytic core subunits (I and II) of cytochrome C oxidase from Rhodobacter sphaeroides with E101A (II) mutation

Released:
DOI: 10.2210/pdb6ci0/pdb
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Source organism: Cereibacter sphaeroides
Primary publication:
The K-path entrance in cytochrome c oxidase is defined by mutation of E101 and controlled by an adjacent ligand binding domain.
Hiser C, Liu J, Ferguson-Miller S
Biochim Biophys Acta Bioenerg 1859 725-733 (2018)
PMID: 29626419

Function and Biology Details

Reaction catalysed: 
4 ferrocytochrome c + O(2) + 8 H(+)(Side 1) = 4 ferricytochrome c + 4 H(2)O(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152619 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Cytochrome c oxidase subunit 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 535 amino acids
Theoretical weight: 59.54 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides 2.4.1
UniProt:
  • Canonical: P33517 (Residues: 17-551; Coverage: 95%)
Gene name: ctaD
Sequence domains: Cytochrome C and Quinol oxidase polypeptide I
Structure domains: Cytochrome c oxidase-like, subunit I domain
Cytochrome c oxidase subunit 2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 257 amino acids
Theoretical weight: 28.65 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides 2.4.1
UniProt:
  • Canonical: Q03736 (Residues: 29-281; Coverage: 91%)
Gene names: coxII, ctaB, ctaC
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
10 bound ligands:
Ligand DMU 6 x DMU
Ligand TRD 14 x TRD
Ligand CU 6 x CU
Ligand MG 2 x MG
Ligand CA 2 x CA
Ligand HEA 4 x HEA
Ligand CD 2 x CD
Ligand HTH 3 x HTH
Ligand TRS 1 x TRS
Ligand K 1 x K
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 124.916Å b: 130.969Å c: 177.788Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.234
Expression system: Cereibacter sphaeroides 2.4.1

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Citations

1 review citation

Interaction of Terminal Oxidases with Amphipathic Molecules.
Azarkina et al. (2023)
5 other articles

1 mention without citation

The K-path entrance in cytochrome c oxidase is defined by mutation of E101 and controlled by an adjacent ligand binding domain.
Hiser et al. (2018)

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