› 6cju

Electron Microscopy
3.35Å resolution

Structure of the SthK cyclic nucleotide-gated potassium channel in complex with cAMP

Released:
DOI: 10.2210/pdb6cju/pdb
PDB entry 6cju coloured by chain, front view.
PDB entry 6cju coloured by chain, side view.
PDB entry 6cju coloured by chain, top view.
Source organism: Spirochaeta thermophila DSM 6578
Primary publication:
Ligand discrimination and gating in cyclic nucleotide-gated ion channels from apo and partial agonist-bound cryo-EM structures.
Rheinberger J, Gao X, Schmidpeter PA, Nimigean CM
Elife 7 (2018)
PMID: 30028291
Related structures: EMD-7484

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123836 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic nucleotide-binding domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 456 amino acids
Theoretical weight: 51.12 KDa
Source organism: Spirochaeta thermophila DSM 6578
Expression system: Escherichia coli
UniProt:
  • Canonical: G0GA88 (Residues: 1-420; Coverage: 98%)
Gene name: Spith_0644
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CMP 4 x CMP
Ligand PGW 32 x PGW
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.35Å
Relevant EMDB volumes: EMD-7484
Expression system: Escherichia coli

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Citations

5 review citations

Advances in high-speed atomic force microscopy (HS-AFM) reveal dynamics of transmembrane channels and transporters.
Heath et al. (2019)
4 more

9 mentions without citation

Structural basis of properties, mechanisms, and channelopathy of cyclic nucleotide-gated channels.
Hu et al. (2023)
8 more