› 6cme

X-ray diffraction
1.92Å resolution

Structure of wild-type ISL2-LID in complex with LHX4-LIM1+2

Released:
DOI: 10.2210/pdb6cme/pdb
PDB entry 6cme coloured by chain, front view.
PDB entry 6cme coloured by chain, side view.
PDB entry 6cme coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Mutation in a flexible linker modulates binding affinity for modular complexes.
Stokes PH, Robertson NO, Silva APG, Estephan T, Trewhella J, Guss JM, Matthews JM
Proteins 87 425-429 (2019)
PMID: 30788856

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156872 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Insulin gene enhancer protein ISL-2; LIM/homeobox protein Lhx4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 18.75 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53776 (Residues: 24-149; Coverage: 32%)
  • Canonical: Q9CXV0 (Residues: 153-159; Coverage: 2%)
Gene names: Gsh-4, Gsh4, Isl2, Lhx4
Sequence domains: LIM domain
Structure domains: Cysteine Rich Protein

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 47.488Å b: 88.843Å c: 50.303Å
α: 90° β: 111.33° γ: 90°
R-values:
R R work R free
0.203 0.201 0.243
Expression system: Escherichia coli BL21(DE3)

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Citations

2 mentions without citation

Bioinformatic Analysis of Structure and Function of LIM Domains of Human Zyxin Family Proteins.
Siddiqui et al. (2021)
1 more