› 6com

X-ray diffraction
2.3Å resolution

2.3A crystal structure of E. coli phosphoenolpyruvate carboxykinase mutant Asp269Asn

Released:
DOI: 10.2210/pdb6com/pdb
PDB entry 6com coloured by chain, front view.
PDB entry 6com coloured by chain, side view.
PDB entry 6com coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Kinetic and structural analysis of Escherichia coli phosphoenolpyruvate carboxykinase mutants.
Sokaribo A, Novakovski BAA, Cotelesage J, White AP, Sanders D, Goldie H
Biochim Biophys Acta Gen Subj 1864 129517 (2020)
PMID: 31911238

Function and Biology Details

Reaction catalysed: 
ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149561 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase (ATP) Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 59.71 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P22259 (Residues: 1-540; Coverage: 100%)
Gene names: JW3366, b3403, pck, pckA
Sequence domains: Phosphoenolpyruvate carboxykinase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ATP 1 x ATP
Ligand PYR 1 x PYR
Ligand CA 1 x CA
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: C2
Unit cell:
a: 124.595Å b: 94.175Å c: 46.457Å
α: 90° β: 94.77° γ: 90°
R-values:
R R work R free
0.154 0.148 0.209
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Metabolic Activation of CsgD in the Regulation of Salmonella Biofilms.
Sokaribo et al. (2020)