› 6cum

X-ray diffraction
1.6Å resolution

Crystal structure of a C-terminal proteolytic fragment of a protein annotated as an LAO/AO transport system ATPase but likely MeaB and MMAA-like GTPase from Mycobacterium smegmatis

Released:
DOI: 10.2210/pdb6cum/pdb
PDB entry 6cum coloured by chain, front view.
PDB entry 6cum coloured by chain, side view.
PDB entry 6cum coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Ab initio structure solution of a proteolytic fragment using ARCIMBOLDO.
Abendroth J, Sankaran B, Myler PJ, Lorimer DD, Edwards TE
Acta Crystallogr F Struct Biol Commun 74 530-535 (2018)
PMID: 30198884

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106414 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AAA+ ATPase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 32.67 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0R1T8 (Residues: 1-294; Coverage: 100%)
Gene name: MSMEG_4869
Sequence domains: Methylmalonyl Co-A mutase-associated GTPase MeaB

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3121
Unit cell:
a: 48.53Å b: 48.53Å c: 54.82Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.174 0.208
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

2 mentions without citation

Introduction to molecular replacement: a time perspective.
Dodson E. (2021)
1 more