› 6cuq

X-ray diffraction
2.45Å resolution

Crystal structure of Macrophage migration inhibitory factor-like protein (EhMIF) from Entamoeba histolytica

Released:
DOI: 10.2210/pdb6cuq/pdb
PDB entry 6cuq coloured by chain, front view.
PDB entry 6cuq coloured by chain, side view.
PDB entry 6cuq coloured by chain, top view.
Source organism: Entamoeba histolytica
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111307 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor-like protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 121 amino acids
Theoretical weight: 13.35 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: C4LV00 (Residues: 1-113; Coverage: 100%)
Gene name: EHI_092370
Sequence domains: Macrophage migration inhibitory factor (MIF)

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2221
Unit cell:
a: 77.61Å b: 100.06Å c: 98.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.178 0.225
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links