› 6cux

X-ray diffraction
4.1Å resolution

Escherichia coli RpoB S531L mutant RNA polymerase holoenzyme in complex with Kanglemycin A

Released:
DOI: 10.2210/pdb6cux/pdb
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PDB entry 6cux coloured by chain, front view.
PDB entry 6cux coloured by chain, side view.
PDB entry 6cux coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Mode of Action of Kanglemycin A, an Ansamycin Natural Product that Is Active against Rifampicin-Resistant Mycobacterium tuberculosis.
Mosaei H, Molodtsov V, Kepplinger B, Harbottle J, Moon CW, Jeeves RE, Ceccaroni L, Shin Y, Morton-Laing S, Marrs ECL, Wills C, Clegg W, Yuzenkova Y, Perry JD, Bacon J, Errington J, Allenby NEE, Hall MJ, Murakami KS, Zenkin N
OpenAccess logo Mol Cell 72 263-274.e5 (2018)
PMID: 30244835

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132730 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B, G, H
Molecule details ›
Chains: A, B, G, H
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-329; Coverage: 100%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit beta Chains: C, I
Molecule details ›
Chains: C, I
Length: 1342 amino acids
Theoretical weight: 150.85 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chains: D, J
DNA-directed RNA polymerase subunit omega Chains: E, K
RNA polymerase sigma factor RpoD Chains: F, L

Ligands and Environments

3 bound ligands:
Ligand KNG 1 x KNG
Ligand MG 2 x MG
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 188.167Å b: 204.685Å c: 311.577Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.255
Expression system: Escherichia coli

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Citations

8 review citations

Diverse and unified mechanisms of transcription initiation in bacteria.
Chen et al. (2021)
7 more

2 mentions without citation

Evolving MRSA: High-level β-lactam resistance in Staphylococcus aureus is associated with RNA Polymerase alterations and fine tuning of gene expression.
Panchal et al. (2020)
1 more

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