› 6cwv

X-ray diffraction
1.98Å resolution

Protein Tyrosine Phosphatase 1B A122S mutant

Released:
DOI: 10.2210/pdb6cwv/pdb
PDB entry 6cwv coloured by chain, front view.
PDB entry 6cwv coloured by chain, side view.
PDB entry 6cwv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Evolutionarily Conserved Allosteric Communication in Protein Tyrosine Phosphatases.
Hjortness MK, Riccardi L, Hongdusit A, Zwart PH, Sankaran B, De Vivo M, Fox JM
Biochemistry 57 6443-6451 (2018)
PMID: 30289703

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 38.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-321; Coverage: 74%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P3121
Unit cell:
a: 89.434Å b: 89.434Å c: 105.554Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.246
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Harnessing Conformational Plasticity to Generate Designer Enzymes.
Crean et al. (2020)
1 more

1 mention without citation

Native dynamics and allosteric responses in PTP1B probed by high-resolution HDX-MS.
Woods et al. (2023)