› 6cxx

X-ray diffraction
1.26Å resolution

Horse liver E267H alcohol dehydrogenase complex with 3'-dephosphocoenzyme A

Released:
DOI: 10.2210/pdb6cxx/pdb
PDB entry 6cxx coloured by chain, front view.
PDB entry 6cxx coloured by chain, side view.
PDB entry 6cxx coloured by chain, top view.
Source organism: Equus caballus
Primary publication:
Substitutions of a buried glutamate residue hinder the conformational change in horse liver alcohol dehydrogenase and yield a surprising complex with endogenous 3'-Dephosphocoenzyme A.
Kim YH, Gogerty DS, Plapp BV
Arch Biochem Biophys 653 97-106 (2018)
PMID: 30018019

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-523416 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase E chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 39.86 KDa
Source organism: Equus caballus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00327 (Residues: 2-375; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand COD 2 x COD
Ligand MRD 5 x MRD
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 55.21Å b: 73.08Å c: 181.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.208
Expression system: Escherichia coli

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