› 6cy2

X-ray diffraction
1.4Å resolution

RNA octamer containing 2'-OMe, 4'Calpha-OMe U.

Released:
DOI: 10.2210/pdb6cy2/pdb
Model geometry
Fit model/data
PDB entry 6cy2 coloured by chain, front view.
PDB entry 6cy2 coloured by chain, side view.
PDB entry 6cy2 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structural basis for the synergy of 4'- and 2'-modifications on siRNA nuclease resistance, thermal stability and RNAi activity.
Harp JM, Guenther DC, Bisbe A, Perkins L, Matsuda S, Bommineni GR, Zlatev I, Foster DJ, Taneja N, Charisse K, Maier MA, Rajeev KG, Manoharan M, Egli M
OpenAccess logo Nucleic Acids Res 46 8090-8104 (2018)
PMID: 30107495

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197052 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (5'-R(*(CBV)P*GP*AP*AP*(UOA)P*UP*CP*G)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 8 nucleotides
Theoretical weight: 2.65 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CBV 2 x CBV

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P43212
Unit cell:
a: 31.5Å b: 31.5Å c: 85.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.205
Expression system: Not provided

Quick links

Citations

7 review citations

Current Development of siRNA Bioconjugates: From Research to the Clinic.
Chernikov et al. (2019)
6 more

1 mention without citation

Structural basis for the synergy of 4'- and 2'-modifications on siRNA nuclease resistance, thermal stability and RNAi activity.
Harp et al. (2018)