› 6d7y

X-ray diffraction
1.75Å resolution

1.75 Angstrom Resolution Crystal Structure of the Toxic C-Terminal Tip of CdiA from Pseudomonas aeruginosa in Complex with Immune Protein

Released:
DOI: 10.2210/pdb6d7y/pdb
PDB entry 6d7y coloured by chain, front view.
PDB entry 6d7y coloured by chain, side view.
PDB entry 6d7y coloured by chain, top view.
Source organisms:
Primary publication:
A comparative genomics approach identifies contact-dependent growth inhibition as a virulence determinant.
Allen JP, Ozer EA, Minasov G, Shuvalova L, Kiryukhina O, Satchell KJF, Hauser AR
Proc Natl Acad Sci U S A 117 6811-6821 (2020)
PMID: 32156726

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-219533 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 96 amino acids
Theoretical weight: 10.87 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
immune protein Chain: B
Molecule details ›
Chain: B
Length: 155 amino acids
Theoretical weight: 17.93 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P63
Unit cell:
a: 76.579Å b: 76.579Å c: 61.441Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Forest and Trees: Exploring Bacterial Virulence with Genome-wide Association Studies and Machine Learning.
Allen et al. (2021)
3 more

6 mentions without citation

Functional and Structural Diversity of Bacterial Contact-Dependent Growth Inhibition Effectors.
Cuthbert et al. (2022)
5 more