› 6d80

Electron Microscopy
5Å resolution

Cryo-EM structure of the mitochondrial calcium uniporter from N. fischeri bound to saposin

Released:
DOI: 10.2210/pdb6d80/pdb
PDB entry 6d80 coloured by chain, front view.
PDB entry 6d80 coloured by chain, side view.
PDB entry 6d80 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of a fungal mitochondrial calcium uniporter.
Nguyen NX, Armache JP, Lee C, Yang Y, Zeng W, Mootha VK, Cheng Y, Bai XC, Jiang Y
OpenAccess logo Nature 559 570-574 (2018)
PMID: 29995855
Related structures: EMD-7828

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106584 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Saposin A Chains: F, G, H, I, J, K
Molecule details ›
Chains: F, G, H, I, J, K
Length: 81 amino acids
Theoretical weight: 6.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Calcium uniporter protein, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 416 amino acids
Theoretical weight: 47.93 KDa
Source organism: Aspergillus fischeri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1CWT6 (Residues: 75-488; Coverage: 85%)
Gene names: NFIA_105760, mcu
Sequence domains: Mitochondrial calcium uniporter

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5Å
Relevant EMDB volumes: EMD-7828
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

32 review citations

The machineries, regulation and cellular functions of mitochondrial calcium.
Giorgi et al. (2018)
31 more

2 mentions without citation

Cryo-EM structure of a fungal mitochondrial calcium uniporter.
Nguyen et al. (2018)
1 more