6d90

Electron Microscopy
3.2Å resolution

Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1.

Released:

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 85-mer (preferred)
PDBe Complex ID:
PDB-CPX-283029 (preferred)
Entry contents:
79 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (85 distinct):
Large ribosomal subunit protein uL2 Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 28.09 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Best match: G1TT27 (Residues: 1-257)
Gene name: RPL8
Large ribosomal subunit protein uL3 Chain: B
Molecule details ›
Chain: B
Length: 403 amino acids
Theoretical weight: 46.1 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Best match: G1TL06 (Residues: 1-403)
Gene name: RPL3
Large ribosomal subunit protein uL4 Chain: C
Large ribosomal subunit protein uL18 Chain: D
Large ribosomal subunit protein eL6 Chain: E
Large ribosomal subunit protein uL30 Chain: F
Large ribosomal subunit protein eL8 Chain: G
Large ribosomal subunit protein uL6 Chain: H
Large ribosomal subunit protein uL16 Chain: I
Large ribosomal subunit protein uL5 Chain: J
eL13 Chain: L
Large ribosomal subunit protein eL14 Chain: M
Large ribosomal subunit protein eL15 Chain: N
uL13 Chain: O
Large ribosomal subunit protein uL22 Chain: P
Large ribosomal subunit protein uL15/eL18 domain-containing protein Chain: Q
Ribosomal protein L19 Chain: R
Large ribosomal subunit protein eL20 Chain: S
Large ribosomal subunit protein eL21 Chain: T
Large ribosomal subunit protein eL22 Chain: U
Large ribosomal subunit protein uL14 Chain: V
Large ribosomal subunit protein eL24 Chain: W
Large ribosomal subunit protein uL23 Chain: X
Large ribosomal subunit protein uL24 Chain: Y
Large ribosomal subunit protein eL27 Chain: Z
Large ribosomal subunit protein uL15 Chain: a
Large ribosomal subunit protein eL29 Chain: b
Large ribosomal subunit protein eL30 Chain: c
Large ribosomal subunit protein eL31 Chain: d
Large ribosomal subunit protein eL32 Chain: e
Large ribosomal subunit protein eL33 Chain: f
Large ribosomal subunit protein eL34 Chain: g
Large ribosomal subunit protein uL29 Chain: h
Large ribosomal subunit protein eL36 Chain: i
Large ribosomal subunit protein eL37 Chain: j
Large ribosomal subunit protein eL38 Chain: k
Large ribosomal subunit protein eL39 Chain: l
eL40 Chain: m
eL41 Chain: n
Large ribosomal subunit protein eL42 Chain: o
Large ribosomal subunit protein eL43 Chain: p
Large ribosomal subunit protein eL28 Chain: r
60S acidic ribosomal protein P0 Chain: s
Large ribosomal subunit protein uL11 Chain: t
Large ribosomal subunit protein uL1 Chain: K
Small ribosomal subunit protein uS2 Chain: BB
Small ribosomal subunit protein eS1 Chain: CC
Small ribosomal subunit protein uS5 Chain: DD
Small ribosomal subunit protein uS3 Chain: EE
Small ribosomal subunit protein eS4 Chain: FF
Small ribosomal subunit protein uS7 Chain: GG
Small ribosomal subunit protein eS6 Chain: HH
Small ribosomal subunit protein eS7 Chain: II
Small ribosomal subunit protein eS8 Chain: JJ
Small ribosomal subunit protein uS4 Chain: KK
Small ribosomal subunit protein eS10 Chain: LL
Small ribosomal subunit protein uS17 Chain: MM
Small ribosomal subunit protein eS12 Chain: NN
Small ribosomal subunit protein uS15 Chain: OO
Small ribosomal subunit protein uS11 Chain: PP
Small ribosomal subunit protein uS19 Chain: QQ
Small ribosomal subunit protein uS9 Chain: RR
Small ribosomal subunit protein eS17 Chain: SS
Small ribosomal subunit protein uS13 Chain: TT
Small ribosomal subunit protein eS19 Chain: UU
Small ribosomal subunit protein uS10 Chain: VV
Small ribosomal subunit protein eS21 Chain: WW
Small ribosomal subunit protein uS8 Chain: XX
Small ribosomal subunit protein uS12 Chain: YY
40S ribosomal protein S24 Chain: ZZ
Small ribosomal subunit protein eS25 Chain: aa
Small ribosomal subunit protein eS26 Chain: bb
Small ribosomal subunit protein eS27 Chain: cc
Small ribosomal subunit protein eS28 Chain: dd
Small ribosomal subunit protein uS14 Chain: ee
Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein Chain: ff
Ubiquitin-ribosomal protein eS31 fusion protein Chain: gg
Small ribosomal subunit protein RACK1 Chain: hh
Eukaryotic peptide chain release factor subunit 1 Chain: jj
28S ribosomal RNA Chain: 5
5S ribosomal RNA Chain: 7
5.8S ribosomal RNA Chain: 8
18S Ribosomal RNA Chain: 2
P-tRNA Chain: 3
CrPV-IRES Chain: 4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-7834
Expression system: Escherichia coli