› 6dfw

X-ray diffraction
3.2Å resolution

TCR 8F10 in complex with IAg7-p8G9E

Released:
DOI: 10.2210/pdb6dfw/pdb
Model geometry
Fit model/data
PDB entry 6dfw coloured by chain, front view.
PDB entry 6dfw coloured by chain, side view.
PDB entry 6dfw coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
How C-terminal additions to insulin B-chain fragments create superagonists for T cells in mouse and human type 1 diabetes.
Wang Y, Sosinowski T, Novikov A, Crawford F, White J, Jin N, Liu Z, Zou J, Neau D, Davidson HW, Nakayama M, Kwok WW, Gapin L, Marrack P, Kappler JW, Dai S
Sci Immunol 4 (2019)
PMID: 30952805

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-209001 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
H-2 class II histocompatibility antigen, A-D alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 183 amino acids
Theoretical weight: 20.91 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P04228 (Residues: 26-208; Coverage: 79%)
Gene name: H2-Aa
Sequence domains:
Structure domains:
Ig-like domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 221 amino acids
Theoretical weight: 25.29 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q31135 (Residues: 21-217; Coverage: 77%)
Gene name: H2-Ab1
Sequence domains:
8F10 alpha chain Chains: E, G
8F10 beta chain Chains: F, H

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 93.094Å b: 102.044Å c: 136.001Å
α: 90° β: 107.83° γ: 90°
R-values:
R R work R free
0.236 0.233 0.297
Expression systems:
  • Trichoplusia ni
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

11 review citations

A guide to antigen processing and presentation.
Pishesha et al. (2022)
10 more

3 mentions without citation

How C-terminal additions to insulin B-chain fragments create superagonists for T cells in mouse and human type 1 diabetes.
Wang et al. (2019)
2 more

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO