› 6dht

X-ray diffraction
1.42Å resolution

Function and Biology Details

Reaction catalysed: 
Xyloglucan + H(2)O = xyloglucan oligosaccharides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-501182 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xyloglucan-specific endo-beta-1,4-glucanase BoGH9A Chain: A
Molecule details ›
Chain: A
Length: 569 amino acids
Theoretical weight: 63.61 KDa
Source organism: Bacteroides ovatus ATCC 8483
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A7LXT3 (Residues: 21-583; Coverage: 99%)
Gene name: BACOVA_02649
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand IMD 1 x IMD
Ligand CA 2 x CA
Ligand EDO 14 x EDO
Ligand PEG 3 x PEG
Ligand CL 2 x CL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 130.646Å b: 50.406Å c: 95.98Å
α: 90° β: 109° γ: 90°
R-values:
R R work R free
0.168 0.168 0.197
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

If you eat it, or secrete it, they will grow: the expanding list of nutrients utilized by human gut bacteria.
Glowacki et al. (2020)
5 other articles

1 mention without citation

A Cell-Surface GH9 Endo-Glucanase Coordinates with Surface Glycan-Binding Proteins to Mediate Xyloglucan Uptake in the Gut Symbiont Bacteroides ovatus.
Foley et al. (2019)