Sample information
Author description:
E.coli RF1 bound to E.coli 70S ribosome in response to UAU sense A-site codon
Source organism:
Expression system:
Escherichia coli
Validation information
Experimental information
Specimen preparation
Buffer name | pH | Details |
---|---|---|
- | 7 | 20 mM Tris-HCl, 100 mM NH4Cl, 20.5 mM MgCl2, 0.5 mM EDTA, 6 mM beta-mercaptoethanol |
Vitrification
Cryogen | Temperature | Instrument | Humidity | Details |
---|---|---|---|---|
- | - | FEI VITROBOT MARK IV | 100 | - |
Refinement statistics
Structure solution method:
Electron Microscopy
Resolution:
3.7 Å