› 6dur

X-ray diffraction
1.8Å resolution

Citrobacter freundii tyrosine phenol-lyase complexed with L-phenylalanine

Released:
DOI: 10.2210/pdb6dur/pdb
PDB entry 6dur coloured by chain, front view.
PDB entry 6dur coloured by chain, side view.
PDB entry 6dur coloured by chain, top view.
Source organism: Citrobacter freundii
Primary publication:
Crystal Structures of Wild-Type and F448A Mutant Citrobacter freundii Tyrosine Phenol-Lyase Complexed with a Substrate and Inhibitors: Implications for the Reaction Mechanism.
Phillips RS, Craig S
Biochemistry 57 6166-6179 (2018)
PMID: 30260636

Function and Biology Details

Reaction catalysed: 
(1a) L-tyrosine = phenol + 2-aminoprop-2-enoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine phenol-lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 455 amino acids
Theoretical weight: 51.38 KDa
Source organism: Citrobacter freundii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31013 (Residues: 2-456; Coverage: 100%)
Gene name: tpl
Sequence domains: Beta-eliminating lyase

Ligands and Environments

5 bound ligands:
Ligand P71 1 x P71
Ligand K 2 x K
Ligand PGE 1 x PGE
Ligand P33 1 x P33
Ligand P70 1 x P70
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Unit cell:
a: 59.51Å b: 133.33Å c: 142.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 0.193
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Pancreatic Adenocarcinoma: Unconventional Approaches for an Unconventional Disease.
Gromisch et al. (2020)
2 more