› 6duw

X-ray diffraction
2.2Å resolution

Crystal structure of the alpha-N-catenin actin-binding domain H1 mutant

Released:
DOI: 10.2210/pdb6duw/pdb
PDB entry 6duw coloured by chain, front view.
PDB entry 6duw coloured by chain, side view.
PDB entry 6duw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Force-dependent allostery of the α-catenin actin-binding domain controls adherens junction dynamics and functions.
Ishiyama N, Sarpal R, Wood MN, Barrick SK, Nishikawa T, Hayashi H, Kobb AB, Flozak AS, Yemelyanov A, Fernandez-Gonzalez R, Yonemura S, Leckband DE, Gottardi CJ, Tepass U, Ikura M
OpenAccess logo Nat Commun 9 5121 (2018)
PMID: 30504777

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150680 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catenin alpha-2 Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 28.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26232 (Residues: 651-953; Coverage: 27%)
  • Best match: P26232-2 (Residues: 651-905)
Gene names: CAPR, CTNNA2
Sequence domains: Vinculin family
Structure domains: Alpha-catenin/vinculin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P6522
Unit cell:
a: 108.652Å b: 108.652Å c: 133.078Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.202 0.226
Expression system: Escherichia coli BL21

Quick links

Citations

15 review citations

Insight into Mechanobiology: How Stem Cells Feel Mechanical Forces and Orchestrate Biological Functions.
Argentati et al. (2019)
14 more

1 mention without citation

Structural basis of αE-catenin-F-actin catch bond behavior.
Xu et al. (2020)