› 6dzz

Electron Microscopy
3.6Å resolution

Cryo-EM Structure of the wild-type human serotonin transporter in complex with ibogaine and 15B8 Fab in the inward conformation

Released:
DOI: 10.2210/pdb6dzz/pdb
PDB entry 6dzz coloured by chain, front view.
PDB entry 6dzz coloured by chain, side view.
PDB entry 6dzz coloured by chain, top view.
Source organisms:
Primary publication:
Serotonin transporter-ibogaine complexes illuminate mechanisms of inhibition and transport.
Coleman JA, Yang D, Zhao Z, Wen PC, Yoshioka C, Tajkhorshid E, Gouaux E
Nature 569 141-145 (2019)
PMID: 31019304
Related structures: EMD-8943

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211041 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Sodium-dependent serotonin transporter Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 61.01 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P31645 (Residues: 78-617; Coverage: 86%)
Gene names: HTT, SERT, SLC6A4
Sequence domains: Sodium:neurotransmitter symporter family
15B8 antibody heavy chain Chain: B
Molecule details ›
Chain: B
Length: 118 amino acids
Theoretical weight: 12.98 KDa
Source organism: Mus musculus
Expression system: Mus musculus
15B8 antibody light chain Chain: C
Molecule details ›
Chain: C
Length: 110 amino acids
Theoretical weight: 11.78 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand HJM 1 x HJM
Ligand LMT 1 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-8943
Expression systems:
  • Homo sapiens
  • Mus musculus

Quick links

Citations

24 review citations

A guide to plasma membrane solute carrier proteins.
Pizzagalli et al. (2021)
23 more

27 mentions without citation

Computational Dissection of Membrane Transport at a Microscopic Level.
Jiang et al. (2020)
26 more