› 6e0y

X-ray diffraction
2.26Å resolution

A131Q mutant of cyt P460 of Nitrosomonas sp. AL212 with bound NH2OH

Released:
DOI: 10.2210/pdb6e0y/pdb
PDB entry 6e0y coloured by chain, front view.
PDB entry 6e0y coloured by chain, side view.
PDB entry 6e0y coloured by chain, top view.
Source organism: Nitrosomonas sp. AL212
Primary publication:
Controlling a burn: outer-sphere gating of hydroxylamine oxidation by a distal base in cytochrome P460.
Smith MA, Majer SH, Vilbert AC, Lancaster KM
OpenAccess logo Chem Sci 10 3756-3764 (2019)
PMID: 31015919

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P460 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 169 amino acids
Theoretical weight: 18.9 KDa
Source organism: Nitrosomonas sp. AL212
Expression system: Escherichia coli
UniProt:
  • Canonical: F9ZFJ0 (Residues: 28-196; Coverage: 100%)
Gene name: NAL212_0896
Sequence domains: Cytochrome P460
Structure domains: Cytochrome P460

Ligands and Environments

2 bound ligands:
Ligand HEC 4 x HEC
Ligand HOA 4 x HOA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21
Unit cell:
a: 48.031Å b: 80.941Å c: 120.598Å
α: 90° β: 96.03° γ: 90°
R-values:
R R work R free
0.195 0.193 0.242
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Biological and Bioinspired Inorganic N-N Bond-Forming Reactions.
Ferousi et al. (2020)
1 more

1 mention without citation

Outer coordination sphere influences on cofactor maturation and substrate oxidation by cytochrome P460.
Bollmeyer et al. (2023)