› 6e5y

X-ray diffraction
1.5Å resolution

1.50 Angstrom Resolution Crystal Structure of Argininosuccinate Synthase from Bordetella pertussis in Complex with AMP.

Released:
DOI: 10.2210/pdb6e5y/pdb
PDB entry 6e5y coloured by chain, front view.
PDB entry 6e5y coloured by chain, side view.
PDB entry 6e5y coloured by chain, top view.
Source organism: Bordetella pertussis Tohama I
Entry authors: Minasov G, Shuvalova L, Dubrovska I, Cardona-Correa A, Grimshaw S, Kwon K, Anderson WF, Satchell KJF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-182074 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Argininosuccinate synthase Chain: A
Molecule details ›
Chain: A
Length: 448 amino acids
Theoretical weight: 49.65 KDa
Source organism: Bordetella pertussis Tohama I
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7VTJ9 (Residues: 1-445; Coverage: 100%)
Gene names: BP3537, argG
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand AMP 1 x AMP
Ligand CL 4 x CL
Ligand SO4 3 x SO4
Ligand EDO 2 x EDO
Ligand MLA 1 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P42212
Unit cell:
a: 97.287Å b: 97.287Å c: 93.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.179
Expression system: Escherichia coli BL21(DE3)

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