› 6e61

X-ray diffraction
2.51Å resolution

Bacteroides ovatus mixed-linkage glucan utilization locus (MLGUL) SGBP-A in complex with mixed-linkage heptasaccharide

Released:
DOI: 10.2210/pdb6e61/pdb
PDB entry 6e61 coloured by chain, front view.
PDB entry 6e61 coloured by chain, side view.
PDB entry 6e61 coloured by chain, top view.
Source organism: Bacteroides ovatus ATCC 8483
Primary publication:
Surface glycan-binding proteins are essential for cereal beta-glucan utilization by the human gut symbiont Bacteroides ovatus.
Tamura K, Foley MH, Gardill BR, Dejean G, Schnizlein M, Bahr CME, Louise Creagh A, van Petegem F, Koropatkin NM, Brumer H
Cell Mol Life Sci 76 4319-4340 (2019)
PMID: 31062073

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245285 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
mixed-linkage glucan utilization locus (MLGUL) SGBP-B Chains: A, B
Molecule details ›
Chains: A, B
Length: 557 amino acids
Theoretical weight: 62.69 KDa
Source organism: Bacteroides ovatus ATCC 8483
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
2 bound ligands:
Ligand MG 2 x MG
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-3
Spacegroup: P212121
Unit cell:
a: 86.826Å b: 92.943Å c: 155.253Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

If you eat it, or secrete it, they will grow: the expanding list of nutrients utilized by human gut bacteria.
Glowacki et al. (2020)
2 more

2 mentions without citation

Surface glycan-binding proteins are essential for cereal beta-glucan utilization by the human gut symbiont Bacteroides ovatus.
Tamura et al. (2019)
1 more