› 6ea9

X-ray diffraction
2.1Å resolution

Structure of VACV Poxin in post-reactive state with Gp[2'-5']Ap[3']

Released:
DOI: 10.2210/pdb6ea9/pdb
PDB entry 6ea9 coloured by chain, front view.
PDB entry 6ea9 coloured by chain, side view.
PDB entry 6ea9 coloured by chain, top view.
Source organism: Vaccinia virus WR
Primary publication:
Viral and metazoan poxins are cGAMP-specific nucleases that restrict cGAS-STING signalling.
Eaglesham JB, Pan Y, Kupper TS, Kranzusch PJ
OpenAccess logo Nature 566 259-263 (2019)
PMID: 30728498

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poxin Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 220 amino acids
Theoretical weight: 24.74 KDa
Source organism: Vaccinia virus WR
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01225 (Residues: 1-219; Coverage: 100%)
Gene names: B2R, OPG188, VACWR184
Sequence domains: Nucleopolyhedrovirus p26 protein

Ligands and Environments

1 bound ligand:
Ligand 9BG 5 x 9BG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 215.416Å b: 57.147Å c: 133.113Å
α: 90° β: 120.37° γ: 90°
R-values:
R R work R free
0.2 0.199 0.224
Expression system: Escherichia coli

Quick links

Citations

46 review citations

The Cytosolic DNA-Sensing cGAS-STING Pathway in Cancer.
Kwon et al. (2020)
45 more

3 mentions without citation

Viral and metazoan poxins are cGAMP-specific nucleases that restrict cGAS-STING signalling.
Eaglesham et al. (2019)
2 more