› 6eay

X-ray diffraction
3.72Å resolution

Structural Basis for Broad Neutralization of Ebolaviruses by an Antibody Targeting the Glycoprotein Fusion Loop

Released:
DOI: 10.2210/pdb6eay/pdb
PDB entry 6eay coloured by chain, front view.
PDB entry 6eay coloured by chain, side view.
PDB entry 6eay coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for broad neutralization of ebolaviruses by an antibody targeting the glycoprotein fusion loop.
Janus BM, van Dyk N, Zhao X, Howell KA, Soto C, Aman MJ, Li Y, Fuerst TR, Ofek G
OpenAccess logo Nat Commun 9 3934 (2018)
PMID: 30258051

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232128 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
CA45 light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.29 KDa
Source organism: Macaca fascicularis
Expression system: Homo sapiens
Structure domains: Immunoglobulins
CA45 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 237 amino acids
Theoretical weight: 25.17 KDa
Source organism: Macaca fascicularis
Expression system: Homo sapiens
GP2 Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.64 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Homo sapiens
UniProt:
  • Canonical: Q05320 (Residues: 502-637; Coverage: 21%)
Gene name: GP
Sequence domains: Envelope glycoprotein GP2-like, HR1-HR2
GP1 Chain: B
Molecule details ›
Chain: B
Length: 319 amino acids
Theoretical weight: 35.32 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Homo sapiens
UniProt:
  • Canonical: Q05320 (Residues: 32-501; Coverage: 49%)
Gene name: GP
Sequence domains: Filovirus glycoprotein

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: R32
Unit cell:
a: 153.429Å b: 153.429Å c: 335.862Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.26 0.259 0.297
Expression system: Homo sapiens

Quick links

Citations

4 review citations

Therapeutic strategies to target the Ebola virus life cycle.
Hoenen et al. (2019)
3 more

4 mentions without citation

Structural Biology Illuminates Molecular Determinants of Broad Ebolavirus Neutralization by Human Antibodies for Pan-Ebolavirus Therapeutic Development.
Murin et al. (2021)
3 more