› 6eaz

X-ray diffraction
2.5Å resolution

Apo structure of the mitochondrial calcium uniporter protein MICU2

Released:
DOI: 10.2210/pdb6eaz/pdb
PDB entry 6eaz coloured by chain, front view.
PDB entry 6eaz coloured by chain, side view.
PDB entry 6eaz coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of MICU2 and comparison with MICU1 reveal insights into the uniporter gating mechanism.
Kamer KJ, Jiang W, Kaushik VK, Mootha VK, Grabarek Z
Proc Natl Acad Sci U S A 116 3546-3555 (2019)
PMID: 30755530

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183949 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium uptake protein 2, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 379 amino acids
Theoretical weight: 44.55 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8CD10 (Residues: 68-432; Coverage: 85%)
Gene names: Efha1, Micu2

Ligands and Environments

1 bound ligand:
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 112.562Å b: 125.524Å c: 72.145Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Mitochondrial calcium exchange in physiology and disease.
Garbincius et al. (2022)
9 more

3 mentions without citation

Structure and mechanism of the mitochondrial Ca2+ uniporter holocomplex.
Fan et al. (2020)
2 more