› 6elg

X-ray diffraction
1.38Å resolution

Tryptophan Repressor TrpR from E.coli variant M42F T44L T81I S88Y with Indole-3-acetonitrile

Released:
DOI: 10.2210/pdb6elg/pdb
PDB entry 6elg coloured by chain, front view.
PDB entry 6elg coloured by chain, side view.
PDB entry 6elg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A biosensor for the direct visualization of auxin
Herud-Sikimic O, Stiel AC, Kolb M, Shanmugaratnam S, Berendzen KW, Feldhaus C, Hocker B, Juergens G
Nature (2021)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141677 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trp operon repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 116 amino acids
Theoretical weight: 13.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A881 (Residues: 1-108; Coverage: 100%)
Gene names: JW4356, b4393, rtrY, trpR
Sequence domains: Trp repressor protein
Structure domains: TrpR-like

Ligands and Environments

1 bound ligand:
Ligand 3BO 2 x 3BO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 55.014Å b: 63.468Å c: 65.363Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.143 0.176
Expression system: Escherichia coli BL21

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