› 6et7

X-ray diffraction
2.85Å resolution

Activated heterodimer of the bacteriophytochrome regulated diguanylyl cyclase variant - S505V A526V - from Idiomarina species A28L

Released:
DOI: 10.2210/pdb6et7/pdb
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PDB entry 6et7 coloured by chain, front view.
PDB entry 6et7 coloured by chain, side view.
PDB entry 6et7 coloured by chain, top view.
Source organism: Idiomarina sp. A28L
Primary publication:
Asymmetric activation mechanism of a homodimeric red light-regulated photoreceptor.
Gourinchas G, Heintz U, Winkler A
OpenAccess logo Elife 7 (2018)
PMID: 29869984

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123678 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diguanylate cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 685 amino acids
Theoretical weight: 78.2 KDa
Source organism: Idiomarina sp. A28L
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F7RW09 (Residues: 3-683; Coverage: 100%)
Gene name: A28LD_0430
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand LBV 2 x LBV
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P212121
Unit cell:
a: 49.46Å b: 78.42Å c: 443.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.275
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Signal transduction in photoreceptor histidine kinases.
Möglich A. (2019)
2 more

9 mentions without citation

Bacteriophytochromes - from informative model systems of phytochrome function to powerful tools in cell biology.
Gourinchas et al. (2019)
8 more

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