› 6ey6

X-ray diffraction
2.1Å resolution

C-terminal part (residues 315-516) of PorM with the llama nanobody nb130

Released:
DOI: 10.2210/pdb6ey6/pdb
PDB entry 6ey6 coloured by chain, front view.
PDB entry 6ey6 coloured by chain, side view.
PDB entry 6ey6 coloured by chain, top view.
Source organisms:
Primary publication:
Type IX secretion system PorM and gliding machinery GldM form arches spanning the periplasmic space.
Leone P, Roche J, Vincent MS, Tran QH, Desmyter A, Cascales E, Kellenberger C, Cambillau C, Roussel A
OpenAccess logo Nat Commun 9 429 (2018)
PMID: 29382829

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207645 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Por secretion system protein porM/gldM Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 315 amino acids
Theoretical weight: 34.37 KDa
Source organism: Porphyromonas gingivalis
Expression system: Escherichia coli
UniProt:
  • Canonical: B2RLE8 (Residues: 225-516; Coverage: 57%)
Gene names: PGN_1674, porM
Sequence domains:
nb130 Chains: I, J, K, L, M, N, O, P
Molecule details ›
Chains: I, J, K, L, M, N, O, P
Length: 138 amino acids
Theoretical weight: 15.07 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P1
Unit cell:
a: 55.245Å b: 77.179Å c: 156.302Å
α: 90.24° β: 91.76° γ: 97.16°
R-values:
R R work R free
0.182 0.181 0.215
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Bacterial motility: machinery and mechanisms.
Wadhwa et al. (2022)
4 more

6 mentions without citation

An expanded benchmark for antibody-antigen docking and affinity prediction reveals insights into antibody recognition determinants.
Guest et al. (2021)
5 more