6eyu › Citations

Inward H+ pump xenorhodopsin: Mechanism and alternative optogenetic approach.

<div class='caption-title'>Electrogenic properties of XeR.</div>
<div class='caption-title'>Spectroscopic characterization of NsXeR.</div>
<div class='caption-title'>High-performance liquid chromatography measurements.</div>
Shevchenko V, Mager T, Kovalev K, Polovinkin V, Alekseev A, Juettner J, Chizhov I, Bamann C, Vavourakis C, Ghai R, Gushchin I, Borshchevskiy V, Rogachev A, Melnikov I, Popov A, Balandin T, Rodriguez-Valera F, Manstein DJ, Bueldt G, Bamberg E, Gordeliy V
OpenAccess logo Sci Adv 3 e1603187 (2017)
Cited: 48 times
EuropePMC logo PMID: 28948217

Abstract

Generation of an electrochemical proton gradient is the first step of cell bioenergetics. In prokaryotes, the gradient is created by outward membrane protein proton pumps. Inward plasma membrane native proton pumps are yet unknown. We describe comprehensive functional studies of the representatives of the yet noncharacterized xenorhodopsins from Nanohaloarchaea family of microbial rhodopsins. They are inward proton pumps as we demonstrate in model membrane systems, Escherichia coli cells, human embryonic kidney cells, neuroblastoma cells, and rat hippocampal neuronal cells. We also solved the structure of a xenorhodopsin from the nanohalosarchaeon Nanosalina (NsXeR) and suggest a mechanism of inward proton pumping. We demonstrate that the NsXeR is a powerful pump, which is able to elicit action potentials in rat hippocampal neuronal cells up to their maximal intrinsic firing frequency. Hence, inwardly directed proton pumps are suitable for light-induced remote control of neurons, and they are an alternative to the well-known cation-selective channelrhodopsins.

Articles - 6eyu mentioned but not cited (5)

  1. Schizorhodopsins: A family of rhodopsins from Asgard archaea that function as light-driven inward H+ pumps. Inoue K, Tsunoda SP, Singh M, Tomida S, Hososhima S, Konno M, Nakamura R, Watanabe H, Bulzu PA, Banciu HL, Andrei AŞ, Uchihashi T, Ghai R, Béjà O, Kandori H. Sci Adv 6 eaaz2441 (2020)
  2. Crystal structure of schizorhodopsin reveals mechanism of inward proton pumping. Higuchi A, Shihoya W, Konno M, Ikuta T, Kandori H, Inoue K, Nureki O. Proc Natl Acad Sci U S A 118 e2016328118 (2021)
  3. Lokiarchaeota archaeon schizorhodopsin-2 (LaSzR2) is an inward proton pump displaying a characteristic feature of acid-induced spectral blue-shift. Kojima K, Yoshizawa S, Hasegawa M, Nakama M, Kurihara M, Kikukawa T, Sudo Y. Sci Rep 10 20857 (2020)
  4. Algal rhodopsins encoding diverse signal sequence holds potential for expansion of organelle optogenetics. Sushmita K, Sharma S, Singh Kaushik M, Kateriya S. Biophys Physicobiol 20 e201008 (2023)
  5. Membrane Protein Activity Induces Specific Molecular Changes in Nanodiscs Monitored by FTIR Difference Spectroscopy. Baserga F, Vorkas A, Crea F, Schubert L, Chen JL, Redlich A, La Greca M, Storm J, Oldemeyer S, Hoffmann K, Schlesinger R, Heberle J. Front Mol Biosci 9 915328 (2022)


Reviews citing this publication (9)

  1. Light-Controlled Mammalian Cells and Their Therapeutic Applications in Synthetic Biology. Mansouri M, Strittmatter T, Fussenegger M. Adv Sci (Weinh) 6 1800952 (2019)
  2. Optogenetics at the presynapse. Rost BR, Wietek J, Yizhar O, Schmitz D. Nat Neurosci 25 984-998 (2022)
  3. Diversity, Mechanism, and Optogenetic Application of Light-Driven Ion Pump Rhodopsins. Inoue K. Adv Exp Med Biol 1293 89-126 (2021)
  4. Novel optogenetics tool: Gt_CCR4, a light-gated cation channel with high reactivity to weak light. Hososhima S, Shigemura S, Kandori H, Tsunoda SP. Biophys Rev 12 453-459 (2020)
  5. Applications and challenges of rhodopsin-based optogenetics in biomedicine. Zhang H, Fang H, Liu D, Zhang Y, Adu-Amankwaah J, Yuan J, Tan R, Zhu J. Front Neurosci 16 966772 (2022)
  6. History and Perspectives of Ion-Transporting Rhodopsins. Kandori H. Adv Exp Med Biol 1293 3-19 (2021)
  7. Fluorescence Approaches for Characterizing Ion Channels in Synthetic Bilayers. Islam MS, Gaston JP, Baker MAB. Membranes (Basel) 11 857 (2021)
  8. Molecular Biology of Microbial Rhodopsins. Engelhard M. Methods Mol Biol 2501 53-69 (2022)
  9. Opticool: Cutting-edge transgenic optical tools. Fenelon KD, Krause J, Koromila T. PLoS Genet 20 e1011208 (2024)

Articles citing this publication (34)



Quick links