6f2n

X-ray diffraction
1.15Å resolution

Crystal structure of BCII Metallo-beta-lactamase in complex with KDU197

Released:

Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-137499 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P04190 (Residues: 31-257; Coverage: 100%)
Gene name: blm
Sequence domains: Metallo-beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 53.169Å b: 61.668Å c: 69.675Å
α: 90° β: 93.13° γ: 90°
R-values:
R R work R free
0.135 0.134 0.151
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'