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Molecular mechanism of promoter opening by RNA polymerase III.

<div class='caption-title'>Cryo-EM structure of the Pol III-PIC.</div>
<div class='caption-title'>TFIIIB interactions with C34 and C37 in the PIC.</div>
<div class='caption-title'>Protein-DNA contacts in the Pol III PIC.</div>
Vorländer MK, Khatter H, Wetzel R, Hagen WJH, Müller CW
OpenAccess logo Nature 553 295-300 (2018)
Related entries: 6f40, 6f41, 6f44

Cited: 69 times
EuropePMC logo PMID: 29345638

Abstract

RNA polymerase III (Pol III) and transcription factor IIIB (TFIIIB) assemble together on different promoter types to initiate the transcription of small, structured RNAs. Here we present structures of Pol III preinitiation complexes, comprising the 17-subunit Pol III and the heterotrimeric transcription factor TFIIIB, bound to a natural promoter in different functional states. Electron cryo-microscopy reconstructions, varying from 3.7 Å to 5.5 Å resolution, include two early intermediates in which the DNA duplex is closed, an open DNA complex, and an initially transcribing complex with RNA in the active site. Our structures reveal an extremely tight, multivalent interaction between TFIIIB and promoter DNA, and explain how TFIIIB recruits Pol III. Together, TFIIIB and Pol III subunit C37 activate the intrinsic transcription factor-like activity of the Pol III-specific heterotrimer to initiate the melting of double-stranded DNA, in a mechanism similar to that of the Pol II system.

Articles - 6f42 mentioned but not cited (5)

  1. Molecular mechanism of promoter opening by RNA polymerase III. Vorländer MK, Khatter H, Wetzel R, Hagen WJH, Müller CW. Nature 553 295-300 (2018)
  2. Cryo-EM structures of human RNA polymerase III in its unbound and transcribing states. Girbig M, Misiaszek AD, Vorländer MK, Lafita A, Grötsch H, Baudin F, Bateman A, Müller CW. Nat Struct Mol Biol 28 210-219 (2021)
  3. A Tool for Segmentation of Secondary Structures in 3D Cryo-EM Density Map Components Using Deep Convolutional Neural Networks. Mu Y, Sazzed S, Alshammari M, Sun J, He J. Front Bioinform 1 710119 (2021)
  4. Mechanism of RNA polymerase I selection by transcription factor UAF. Baudin F, Murciano B, Fung HKH, Fromm SA, Mattei S, Mahamid J, Müller CW. Sci Adv 8 eabn5725 (2022)
  5. Benchmark dataset for the Voronoi diagram of 3D spherical balls. Song C, Lee M, Choi S, Kim DS. Data Brief 45 108605 (2022)


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