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Crystal structure of Drosophila melanogaster SNF/U2A' complex

X-ray diffraction
1.42Å resolution
PDB entry 6f4j coloured by chain, front view.
PDB entry 6f4j coloured by chain, side view.
PDB entry 6f4j coloured by chain, top view.
Source organism: Drosophila melanogaster
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1
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Assembly Name: Probable U2 small nuclear ribonucleoprotein A' and U1 small nuclear ribonucleoprotein A
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Multimeric state: hetero dimer
Accessible surface area: 13412.21 Å2
Buried surface area: 2153.71 Å2
Dissociation area: 866.45 Å2
Dissociation energy (ΔGdiss): -0.12 kcal/mol
Dissociation entropy (TΔSdiss): 11.21 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-155033
Assembly 2 (preferred)
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Assembly Name: Probable U2 small nuclear ribonucleoprotein A' and U1 small nuclear ribonucleoprotein A
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Multimeric state: hetero dimer
Accessible surface area: 13376.1 Å2
Buried surface area: 1876.08 Å2
Dissociation area: 896.61 Å2
Dissociation energy (ΔGdiss): 3.01 kcal/mol
Dissociation entropy (TΔSdiss): 11.23 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-155033

Macromolecules

Probable U2 small nuclear ribonucleoprotein A'
Chains: A, B
Length: 176 amino acids
Theoretical weight: 20.47 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9V4Q8 (Residues: 1-176; Coverage: 66%)
Gene names: CG1406, U2A
Pfam: Leucine-rich repeat
InterPro:
CATH: Ribonuclease Inhibitor
Probable U2 small nuclear ribonucleoprotein A' in PDB entry 6f4j, assembly 2, front view.
Probable U2 small nuclear ribonucleoprotein A' in PDB entry 6f4j, assembly 2, side view.
Probable U2 small nuclear ribonucleoprotein A' in PDB entry 6f4j, assembly 2, top view.
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U1 small nuclear ribonucleoprotein A
Chains: C, D
Length: 98 amino acids
Theoretical weight: 11.08 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P43332 (Residues: 1-96; Coverage: 44%)
Gene names: CG4528, D25, fs(1)1621, liz, snf
Pfam: RNA recognition motif
InterPro:
CATH: Alpha-Beta Plaits
U1 small nuclear ribonucleoprotein A in PDB entry 6f4j, assembly 2, front view.
U1 small nuclear ribonucleoprotein A in PDB entry 6f4j, assembly 2, side view.
U1 small nuclear ribonucleoprotein A in PDB entry 6f4j, assembly 2, top view.
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