› 6f8z

X-ray diffraction
2.5Å resolution

Structure of the family GH92 alpha-mannosidase BT3130 from Bacteroides thetaiotaomicron

Released:
DOI: 10.2210/pdb6f8z/pdb
PDB entry 6f8z coloured by chain, front view.
PDB entry 6f8z coloured by chain, side view.
PDB entry 6f8z coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron
Primary publication:
Bacteroides thetaiotaomicron generates diverse α-mannosidase activities through subtle evolution of a distal substrate-binding motif.
Thompson AJ, Spears RJ, Zhu Y, Suits MDL, Williams SJ, Gilbert HJ, Davies GJ
OpenAccess logo Acta Crystallogr D Struct Biol 74 394-404 (2018)
PMID: 29717710

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183654 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-1,2-mannosidase, putative Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 727 amino acids
Theoretical weight: 82.79 KDa
Source organism: Bacteroides thetaiotaomicron
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8A325 (Residues: 19-735; Coverage: 100%)
Gene name: BT_3130
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P6222
Unit cell:
a: 272.301Å b: 272.301Å c: 190.047Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.181 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Host glycan utilization within the Bacteroidetes Sus-like paradigm.
Brown et al. (2021)
2 more

3 mentions without citation

Bacteroides thetaiotaomicron generates diverse α-mannosidase activities through subtle evolution of a distal substrate-binding motif.
Thompson et al. (2018)
2 more