› 6f93

X-ray diffraction
2.8Å resolution

Helicobacter pylori serine hydroxymethyl transferase in apo form

Released:
DOI: 10.2210/pdb6f93/pdb
PDB entry 6f93 coloured by chain, front view.
PDB entry 6f93 coloured by chain, side view.
PDB entry 6f93 coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
Structural and functional insight into serine hydroxymethyltransferase from Helicobacter pylori.
Sodolescu A, Dian C, Terradot L, Bouzhir-Sima L, Lestini R, Myllykallio H, Skouloubris S, Liebl U
OpenAccess logo PLoS One 13 e0208850 (2018)
PMID: 30550583

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157392 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 424 amino acids
Theoretical weight: 46.84 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: P56089 (Residues: 1-416; Coverage: 100%)
Gene names: HP_0183, glyA
Sequence domains: Serine hydroxymethyltransferase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 57.349Å b: 87.548Å c: 162.476Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.236
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Dihydrofolate reductase, thymidylate synthase, and serine hydroxy methyltransferase: successful targets against some infectious diseases.
Shamshad et al. (2022)
4 other articles

1 mention without citation

Structural and functional insight into serine hydroxymethyltransferase from Helicobacter pylori.
Sodolescu et al. (2018)